Upinder Singh

Infectious disease doctor

Associate Professor of Medicine (Infectious Diseases & Geographic Medicine) and of Microbiology and Immunology

Professional Education

Residency: University of Virginia Health System (1995) VA

Fellowship: University of Virginia School of Medicine (1998) VA

Internship: University of Virginia Health System (1993) VA

Medical Education: Ohio State University (1992) OH

BS, Ohio State University, Biochemistry (1987)

MD, Ohio State University, Medicine (1992)

Honors & Awards

Member, ASCI (2010)

Administrative Appointments

Division Chief, Infectious Diseases and Geographic Medicine (2010 - Present)

Fellowship Co-Director, Division of Infectious Diseases (2008 - 2013)

Robust gene silencing mediated by antisense small RNAs in the pathogenic protist Entamoeba histolytica
Morf, L., Pearson, R. J., Wang, A. S., & Singh, U. (2013). Robust gene silencing mediated by antisense small RNAs in the pathogenic protist Entamoeba histolytica. NUCLEIC ACIDS RESEARCH, 41(20), 9424-9437.

Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica
Pearson, R. J., Morf, L., & Singh, U. (2013). Regulation of H2O2 Stress-responsive Genes through a Novel Transcription Factor in the Protozoan Pathogen Entamoeba histolytica. JOURNAL OF BIOLOGICAL CHEMISTRY, 288(6), 4462-4474.

Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes
Zhang, H., Ehrenkaufer, G. M., Hall, N., & Singh, U. (2013). Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes. BMC GENOMICS, 14.

Distinct Distal Gut Microbiome Diversity and Composition in Healthy Children from Bangladesh and the United States
Lin, A., Bik, E. M., Costello, E. K., Dethlefsen, L., Haque, R., & Singh, U. (2013). Distinct Distal Gut Microbiome Diversity and Composition in Healthy Children from Bangladesh and the United States. PLOS ONE, 8(1).

The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.
Ehrenkaufer, G. M., Weedall, G. D., Williams, D., Lorenzi, H. A., Caler, E., & Singh, U. (2013). The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation. Genome biology, 14(7), R77.

Distinct distal gut microbiome diversity and composition in healthy children from Bangladesh and the United States.
Lin, A., Bik, E. M., Costello, E. K., Dethlefsen, L., Haque, R., & Singh, U. (2013). Distinct distal gut microbiome diversity and composition in healthy children from Bangladesh and the United States. PloS one, 8(1).

The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation
Ehrenkaufer, G. M., Weedall, G. D., Williams, D., Lorenzi, H. A., Caler, E., & Singh, U. (2013). The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation. GENOME BIOLOGY, 14(7).

Oxidative stress resistance genes contribute to the pathogenic potential of the anaerobic protozoan parasite, Entamoeba histolytica
Rastew, E., Vicente, J. B., & Singh, U. (2012). Oxidative stress resistance genes contribute to the pathogenic potential of the anaerobic protozoan parasite, Entamoeba histolytica. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 42(11), 1007-1015.

A Detoxifying Oxygen Reductase in the Anaerobic Protozoan Entamoeba histolytica
Vicente, J. B., Vy Tran, V., Pinto, L., Teixeira, M., & Singh, U. (2012). A Detoxifying Oxygen Reductase in the Anaerobic Protozoan Entamoeba histolytica. EUKARYOTIC CELL, 11(9), 1112-1118.

Entamoeba histolytica: a snapshot of current research and methods for genetic analysis
Morf, L., & Singh, U. (2012). Entamoeba histolytica: a snapshot of current research and methods for genetic analysis. CURRENT OPINION IN MICROBIOLOGY, 15(4), 469-475.

Transient and stable transfection in the protozoan parasite Entamoeba invadens
Ehrenkaufer, G. M., & Singh, U. (2012). Transient and stable transfection in the protozoan parasite Entamoeba invadens. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 184(1), 59-62.

Nucleus-localized Antisense Small RNAs with 5 '-Polyphosphate Termini Regulate Long Term Transcriptional Gene Silencing in Entamoeba histolytica G3 Strain
Zhang, H., Alramini, H., Vy Tran, V., & Singh, U. (2011). Nucleus-localized Antisense Small RNAs with 5 '-Polyphosphate Termini Regulate Long Term Transcriptional Gene Silencing in Entamoeba histolytica G3 Strain. JOURNAL OF BIOLOGICAL CHEMISTRY, 286(52), 44467-44479.

Antiparasitic Therapy
Kappagoda, S., Singh, U., & Blackburn, B. G. (2011). Antiparasitic Therapy. MAYO CLINIC PROCEEDINGS, 86(6), 561-583.

RNA interference in Entamoeba histolytica: implications for parasite biology and gene silencing
Zhang, H., Pompey, J. M., & Singh, U. (2011). RNA interference in Entamoeba histolytica: implications for parasite biology and gene silencing. FUTURE MICROBIOLOGY, 6(1), 103-117.

Approaches to characterizing Entamoeba histolytica transcriptional regulation
Pearson, R. J., & Singh, U. (2010). Approaches to characterizing Entamoeba histolytica transcriptional regulation. CELLULAR MICROBIOLOGY, 12(12), 1681-1690.

Downregulation of an Entamoeba histolytica Rhomboid Protease Reveals Roles in Regulating Parasite Adhesion and Phagocytosis
Baxt, L. A., Rastew, E., Bracha, R., Mirelman, D., & Singh, U. (2010). Downregulation of an Entamoeba histolytica Rhomboid Protease Reveals Roles in Regulating Parasite Adhesion and Phagocytosis. EUKARYOTIC CELL, 9(8), 1283-1293.

A developmentally regulated Myb domain protein regulates expression of a subset of stage-specific genes in Entamoeba histolytica
Ehrenkaufer, G. M., Hackney, J. A., & Singh, U. (2009). A developmentally regulated Myb domain protein regulates expression of a subset of stage-specific genes in Entamoeba histolytica. CELLULAR MICROBIOLOGY, 11(6), 898-910.

A Pseudouridine Synthase Homologue Is Critical to Cellular Differentiation in Toxoplasma gondii
Anderson, M. Z., Brewer, J., Singh, U., & Boothroyd, J. C. (2009). A Pseudouridine Synthase Homologue Is Critical to Cellular Differentiation in Toxoplasma gondii. EUKARYOTIC CELL, 8(3), 398-409.

Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica
Linford, A. S., Moreno, H., Good, K. R., Zhang, H., Singh, U., & Petri, W. A. (2009). Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica. BMC MICROBIOLOGY, 9.

Recent insights into Entamoeba development: Identification of transcriptional networks associated with stage conversion
Singh, U., & Ehrenkaufer, G. M. (2009). Recent insights into Entamoeba development: Identification of transcriptional networks associated with stage conversion. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 39(1), 41-47.

Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis
Vicente, J. B., Ehrenkaufer, G. M., Saraiva, L. M., Teixeira, M., & Singh, U. (2009). Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis. CELLULAR MICROBIOLOGY, 11(1), 51-69.

Small RNAs with 5 '- Polyphosphate Termini Associate with a Piwi-Related Protein and Regulate Gene Expression in the Single-Celled Eukaryote Entamoeba histolytica
Zhang, H., Ehrenkaufer, G. M., Pompey, J. M., Hackney, J. A., & Singh, U. (2008). Small RNAs with 5 '- Polyphosphate Termini Associate with a Piwi-Related Protein and Regulate Gene Expression in the Single-Celled Eukaryote Entamoeba histolytica. PLOS PATHOGENS, 4(11).

Transcriptional Regulatory Networks in Entamoeba histolytica
Ehrenkaufer, G. M., & Singh, U. (2008). Transcriptional Regulatory Networks in Entamoeba histolytica. CURRENT DRUG TARGETS, 9(11), 931-937.

New insights into Entamoeba histolytica pathogenesis
Baxt, L. A., & Singh, U. (2008). New insights into Entamoeba histolytica pathogenesis. CURRENT OPINION IN INFECTIOUS DISEASES, 21(5), 489-494.

An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion
Baxt, L. A., Baker, R. P., Singh, U., & Urban, S. (2008). An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion. GENES & DEVELOPMENT, 22(12), 1636-1646.

Loss of dsRNA-based gene silencing in Entamoeba histolytica: Implications for approaches to genetic analysis
MacFarlane, R. C., & Singh, U. (2008). Loss of dsRNA-based gene silencing in Entamoeba histolytica: Implications for approaches to genetic analysis. EXPERIMENTAL PARASITOLOGY, 119(2), 296-300.

Identification of an Entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity
MacFarlane, R. C., & Singh, U. (2007). Identification of an Entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity. EUKARYOTIC CELL, 6(11), 2139-2146.

Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica
Ehrenkaufer, G. M., Eichinger, D. J., & Singh, U. (2007). Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica. BMC GENOMICS, 8.

Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite
Ehrenkaufer, G. M., Haque, R., Hackney, J. A., Eichinger, D. J., & Singh, U. (2007). Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite. CELLULAR MICROBIOLOGY, 9(6), 1426-1444.

Functional characterization of spliceosomal introns and identification of U2, U4, and U5 snRNAs in the deep-branching eukaryote Entamoeba histolytica
Davis, C. A., Brown, M. Ps., & Singh, U. (2007). Functional characterization of spliceosomal introns and identification of U2, U4, and U5 snRNAs in the deep-branching eukaryote Entamoeba histolytica. EUKARYOTIC CELL, 6(6), 940-948.

Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference
Hackney, J. A., Ehrenkaufer, G. M., & Singh, U. (2007). Identification of putative transcriptional regulatory networks in Entamoeba histolytica using Bayesian inference. NUCLEIC ACIDS RESEARCH, 35(7), 2141-2152.

Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression
Ali, I. K. M., Ehrenkaufer, G. M., Hackney, J. A., & Singh, U. (2007). Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression. BMC GENOMICS, 8.

Structure and content of the Entamoeba histolytica genome
Clark, C. G., Alsmark, U. Cm., Tazreiter, M., Saito-Nakano, Y., Ali, V., & Hall, N. (2007). Structure and content of the Entamoeba histolytica genome. ADVANCES IN PARASITOLOGY, VOL 65, 65, 51-190.

Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome
Gilchrist, C. A., Houpt, E., Trapaidze, N., Fei, Z., Crasta, O., & Petri, W. A. (2006). Impact of intestinal colonization and invasion on the Entamoeba histolytica transcriptome. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 147(2), 163-176.

Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: Potential implications for amebic pathogenesis
MacFarlane, R. C., & Singh, U. (2006). Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: Potential implications for amebic pathogenesis. INFECTION AND IMMUNITY, 74(1), 340-351.

Disruption of a locus encoding a nucleolar zinc finger protein decreases tachyzoite-to-bradyzoite differentiation in Toxoplasma gondii
Vanchinathan, P., Brewer, J. L., Harb, O. S., Boothroyd, J. C., & Singh, U. (2005). Disruption of a locus encoding a nucleolar zinc finger protein decreases tachyzoite-to-bradyzoite differentiation in Toxoplasma gondii. INFECTION AND IMMUNITY, 73(10), 6680-6688.

Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: Implications for epidemiological studies
Bhattacharya, D., Haque, R., & Singh, U. (2005). Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: Implications for epidemiological studies. JOURNAL OF CLINICAL MICROBIOLOGY, 43(9), 4815-4819.

Genomic DNA microarrays for Entamoeba histolytica: Applications for use in expression profiling and strain genotyping
Macfarlane, R., Bhattacharya, D., & Singh, U. (2005). Genomic DNA microarrays for Entamoeba histolytica: Applications for use in expression profiling and strain genotyping. EXPERIMENTAL PARASITOLOGY, 110(3), 196-202.

Transcriptional profiling of Entamoeba histolytica trophozoites
MacFarlane, R. C., Shah, P. H., & Singh, U. (2005). Transcriptional profiling of Entamoeba histolytica trophozoites. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 35(5), 533-542.

Comparative genomic hybridizations of Entamoeba strains reveal unique genetic fingerprints that correlate with virulence
Shah, P. H., MacFarlane, R. C., Bhattacharya, D., Matese, J. C., Demeter, J., & Singh, U. (2005). Comparative genomic hybridizations of Entamoeba strains reveal unique genetic fingerprints that correlate with virulence. EUKARYOTIC CELL, 4(3), 504-515.

The genome of the protist parasite Entamoeba histolytica
Loftus, B., Anderson, I., Davies, R., Alsmark, U. Cm., Samuelson, J., & Hall, N. (2005). The genome of the protist parasite Entamoeba histolytica. NATURE, 433(7028), 865-868.

DNA content analysis on microarrays.
Singh, U., Shah, P. H., & MacFarlane, R. C. (2004). DNA content analysis on microarrays. Methods in molecular biology (Clifton, N.J.), 270, 237-248.

DNA microarrays in parasitology: strengths and limitations
Boothroyd, J. C., Blader, I., Cleary, M., & Singh, U. (2003). DNA microarrays in parasitology: strengths and limitations. TRENDS IN PARASITOLOGY, 19(10), 470-476.

Toxoplasma gondii asexual development: Identification of developmentally regulated genes and distinct patterns of gene expression
Cleary, M. D., Singh, U., Blader, I. J., Brewer, J. L., & Boothroyd, J. C. (2002). Toxoplasma gondii asexual development: Identification of developmentally regulated genes and distinct patterns of gene expression. EUKARYOTIC CELL, 1(3), 329-340.

Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction
Singh, U., Brewer, J. L., & Boothroyd, J. C. (2002). Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction. MOLECULAR MICROBIOLOGY, 44(3), 721-733.

Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly
Singh, U., Gilchrist, C. A., Schaenman, J. M., Rogers, J. B., Hockensmith, J. W., & Petri, W. A. (2002). Context-dependent roles of the Entamoeba histolytica core promoter element GAAC in transcriptional activation and protein complex assembly. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 120(1), 107-116.

Diagnosis and management of amebiasis
Petri, W. A., & Singh, U. (1999). Diagnosis and management of amebiasis. CLINICAL INFECTIOUS DISEASES, 29(5), 1117-1125.

Diagnosis and management of amebiasis.
Singh, U., Petri, Jr, & WA. (1999). Diagnosis and management of amebiasis. Clinical Infectious Diseases., (29).

The novel core promoter element GAAC in the hgl5 gene of Entamoeba histolytica is able to direct a transcription start site independent of TATA or Inr regions.
Singh U, & Rogers J. (1998). The novel core promoter element GAAC in the hgl5 gene of Entamoeba histolytica is able to direct a transcription start site independent of TATA or Inr regions. Journal of Biological Chemistry., (273).

Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica
Singh, U., Rogers, J. B., Mann, B. J., & Petri, W. A. (1997). Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 94(16), 8812-8817.